O’GeneRuler™ Express DNA Ladder, ready-to-use
DNA ladder for fast sizing and approximate quantification of a wide range of double-stranded DNA on agarose gels. The ladder is premixed with blue and yellow tracking dyes.
Detailní popis
Thermo Scientific O'GeneRuler Express DNA Ladder 100-5000 bp, ready-to-use is designed for fast separation (in 5 to 15 minutes at 23 V/cm) of double-stranded DNA fragments in agarose gels (see Table 1). The band pattern of express ladders is not affected by the composition of electrophoresis buffer, voltage, or gel percentage. The ladder is a mixture of chromatography-purified individual DNA fragments.
O'GeneRuler Express DNA Ladder, ready-to-use is premixed with 6X Orange DNA Loading Dye for direct loading on gel. 6X Orange DNA Loading Dye contains xylene cyanol FF and orange G. In a 1% agarose gel, orange G dye migrates at 50 bp.
The ladder is supplied with 6X Orange DNA Loading Dye for sample DNA.
Highlights
- Ideal for both DNA sizing and approximate quantification
- Ideal for fast separation under a wide range of electrophoresis conditions
- Sharp bands
- Bright reference bands (given in red)
- Can be directly loaded and are stable at room temperature for 6 months
- Supplied with loading dye for sample DNA
| 6X Orange DNA Loading Dye | 10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA. |
| Hazardous | No |
| O'GeneRuler™ Storage and Loading Buffer | 10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, and 10% glycerol. |
| Quality Control |
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| Storage | Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C. |
| Storage Condition | -20 C |
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