NTP Set, 100 mM Solutions
Kód produktu: R0481
Kód výrobce: R0481
Kód dodavatele: {37605287-5B31-4642-B1D0-EA49ABC48908}
Výrobce:
Life Technologies Czech Republic s.r.o.
Formula: C40H30N10O6Cl2, Molecular weight: 817.7
NBT (nitro blue tetrazolium) is an oxidant used in conjunction with BCIP or BCIP derivatives (BCIP T) to enhance blue color development during detection of alkaline phosphatase activity.
Detailní popis
Thermo Scientific NBT is used to enhance blue color development during detection of alkaline phosphatase activity (see Protocols). It is suitable for immunohistochemistry and cytochemistry. NBT is also a substrate for oxidases and dehydrogenases.
Applications
- Detection of alkaline phosphatase activity in:
- Southern blotting
- Western blotting
- Northern blotting
- Dot/slot blots
- Plaque and colony screening
NBT
Formula: C40H30N10O6Cl2
Molecular weight: 817.7
Note
Preparation of a 75 g/mL stock solution in 70% dimethylformamide is recommended.
| Formula | C40H30N10O6Cl2 |
| Hazardous | No |
| Molecular weight | 817.7 |
| Quality Control | One spot by TLC. Less than 8% water content. |
| Storage | Store at 4°C or at -20°C in the dark. |
| Storage Condition | 4 C |
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