NTP Set, 100 mM Solutions

Kód produktu: R0481 Kód výrobce: R0481 Kód dodavatele: {37605287-5B31-4642-B1D0-EA49ABC48908} Výrobce: UAB Fermentas
3 448,50 Kč
2 850,00 Kč bez DPH
do týdne
4x0,25 ml

Formula: C40H30N10O6Cl2, Molecular weight: 817.7

NBT (nitro blue tetrazolium) is an oxidant used in conjunction with BCIP or BCIP derivatives (BCIP T) to enhance blue color development during detection of alkaline phosphatase activity.
Zobraz detailní popis

Detailní popis

Thermo Scientific NBT is used to enhance blue color development during detection of alkaline phosphatase activity (see Protocols). It is suitable for immunohistochemistry and cytochemistry. NBT is also a substrate for oxidases and dehydrogenases.

Applications

  • Detection of alkaline phosphatase activity in:
    • Southern blotting
    • Western blotting
    • Northern blotting
    • Dot/slot blots
    • Plaque and colony screening
NBT Chemical Structure

NBT
Formula:
C40H30N10O6Cl2
Molecular weight: 817.7

Note

Preparation of a 75 g/mL stock solution in 70% dimethylformamide is recommended.

Formula C40H30N10O6Cl2
Hazardous No
Molecular weight 817.7
Quality Control One spot by TLC. Less than 8% water content.
Storage Store at 4°C or at -20°C in the dark.
Storage Condition 4 C

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