Thermo Scientific NBT is used to enhance blue color development during detection of alkaline phosphatase activity (see Protocols). It is suitable for immunohistochemistry and cytochemistry. NBT is also a substrate for oxidases and dehydrogenases.
- Detection of alkaline phosphatase activity in:
- Southern blotting
- Western blotting
- Northern blotting
- Dot/slot blots
- Plaque and colony screening
Preparation of a 75 g/mL stock solution in 70% dimethylformamide is recommended.
|Quality Control||One spot by TLC. Less than 8% water content.|
|Storage||Store at 4°C or at -20°C in the dark.|
|Storage Condition||4 C|
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