Tento e-shop využívá cookies

Na našich webových stránkách používáme soubory cookies. Některé z nich jsou nezbytné, zatímco jiné nám pomáhají vylepšit tento web a váš uživatelský zážitek. Souhlasíte s používáním všech cookies?

Cookies nastavení

Vaše soukromí je důležité. Můžete si vybrat nastavení cookies níže.

MnlI

Kód produktu: ER1071 Kód výrobce: ER1071 Kód dodavatele: {4E07699D-CC97-46AA-8F2C-AB9DF679E742} Výrobce: Life Technologies Czech Republic s.r.o.
2 078,78 Kč
1 718,00 Kč bez DPH
do týdne
300 Units

5'...C C T C (N)7...3'
3'...G G A G (N)6...5'

Zobraz detailní popis

Detailní popis

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

MnlI produces DNA fragments that have a single-base 3 extension which are more difficult to ligate than blunt-ended fragments. MnlI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.

For methylation sensitivity refer to product specifications.

Conditions for 100% Activity
  • 1X Buffer G: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl, and 0.1mg/mL BSA
  • Incubate at 37°C
Double Digestion Perform double digestion using DoubleDigest.
Hazardous No
Isoschizomers Search for commercial isoschizomers using REsearch.
Storage Buffer MnlI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, and 50% (v/v) glycerol
Storage Condition -20 C

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X
G (green)
1X
O (orange)
1X
R (red)
1X
Tango
(yellow)
1X / 2X
G (Green) 37°C 50-100 100 20-50 20-50 20-50 20-50 1X or 2X

Methylation Effects

  • Dam: never overlaps - no effect.
  • Dcm: never overlaps - no effect.
  • CpG: may overlap - no effect.
  • EcoKI: never overlaps - no effect.
  • EcoBI: may overlap - blocked.
Methylation type Sequence Cleavage effect
CpG 5'...CCTm5C G...3'
3'...GGA Gm5C...5'
No effect
EcoBI(TGA(N)8TGCT) 5'...TGm6AGG(N)6 TGCT...3'
3'...AC TCC(N)6m6ACGA...5'
Blocked

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
0 50-100

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
262 34 61 26 13 14
pTZ19R/U pTZ57R pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
12 13 14 14 26 27

Hodnocení produktu

Produkt zatím nikdo nehodnotil, buďte první!