Tento e-shop využívá cookies

Na našich webových stránkách používáme soubory cookies. Některé z nich jsou nezbytné, zatímco jiné nám pomáhají vylepšit tento web a váš uživatelský zážitek. Souhlasíte s používáním všech cookies?

Nastavení Jen nezbytné Souhlasím
Cookies nastavení

Vaše soukromí je důležité. Můžete si vybrat nastavení cookies níže.

Potvrzuji
  1. Úvodní strana
  2. Reagencie
  3. Life Technologies / Fermentas
  4. PCR, qPCR, RT-PCR & dNTPs
  5. Maxima Hot Start PCR Master Mix (2X)

Maxima Hot Start PCR Master Mix (2X)

Kód produktu: K1052 Kód výrobce: K1052 Kód dodavatele: {7985EC37-65EA-4544-BE26-12DAC3AAB370} Výrobce: UAB Fermentas
Maxima Hot Start PCR Master Mix (2X)
11 688,60 Kč
9 660,00 Kč bez DPH
500 rxns

Store at -20°C Not inactivated at 80°C in 20 min
Hot start DNA polymerase designed to enhance the specificity, sensitivity, and yield of DNA amplification.
Zobraz detailní popis

Detailní popis

Thermo Scientific Maxima Hot Start Taq DNA Polymerase is a chemically modified recombinant Taq DNA polymerase. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is restored during a short 4 minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase. Maxima Hot Start Taq DNA Polymerase is also available in a master mix format.

Highlights

  • Four minute activation time
  • High PCR specificity and sensitivity
  • Room temperature PCR set up

Applications

  • Hot Start PCR
  • Routine PCR
  • High throughput PCR
  • Multiplex PCR
  • Genotyping

Includes

Maxima Hot Start Taq DNA Polymerase:

  • Maxima Hot Start Taq DNA Polymerase (5 U/µL)
  • 10X Hot Start PCR Buffer
  • 25 mM MgCl2

Maxima Hot Start PCR Master Mix (2X):

  • Maxima Hot Start Taq DNA polymerase, 2X Hot Start PCR buffer, 0.4 mM of each dNTP and 4 mM Mg2+.
  • Nuclease-free water
10X Hot Start PCR Buffer 200 mM Tris-HCl (pH 8.3 at 25°C), 200 mM KCl, 50 mM (NH4)2SO4.
Definition of Activity Unit
  • One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 74°C.
  • Enzyme activity is assayed in the following mixture: 25 mM TAPS (pH 9.3 at 25°C), 50 mM KCl, 2 mM MgCl2, 0.2 mM of each dATP, dGTP, dTTP, 0.1 mM dCTP, 0.75 mM activated salmon milt DNA, and 0.4 MBq/mL [3H]-dCTP.
Hazardous No
Inactivation Inactivated by phenol/chloroform extraction.
Inhibition Inhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02 and 0.01%, respectively (1).
Molecular Weight 94 kDa monomer
Quality Control
  • The absence of endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.
  • Functionally tested in hot-start PCR.
Storage Buffer The enzyme is supplied in 20 mM Tris-HCl (pH 9.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Tween 20, 0.5% (v/v) Nonidet P40, and 50% (v/v) glycerol.
Storage Condition -20 C

Hodnocení produktu

Produkt zatím nikdo nehodnotil, buďte první!