MagJET Whole Blood gDNA Kit

Detailní popis

The Thermo Scientific MagJET Whole Blood Genomic DNA Kit is designed for fast and efficient purification of genomic DNA from fresh or frozen whole blood treated with EDTA or citrate, as well as blood fractions. Note: Samples preserved with heparin are not compatible with this kit. 

The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET magnetic beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual purification by low sample throughput users.

The resulting high quality DNA is free of proteins, nucleases and other contaminants or inhibitors, can be used in a wide range of downstream applications such as PCR, qPCR and other enzymatic reactions.

Highlights

• Compatible – suitable to use with common stabilizers such as EDTA, citrates
• Versatile – suitable for gDNA purification from fresh, frozen or dried blood, as well as bone marrow and buffy coat
• Efficient – high yields of pure DNA free of contaminants and downstream application inhibitors
• Flexible – easily adjustable for high throughput automation platforms and manual processing

Applications

Isolated genomic DNA is ideal for use in common molecular biology procedures, including:

• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting

Includes

• Lysis Buffer
• MagJET Magnetic Beads
• Proteinase K
• Binding Buffer
• Wash Buffer 1 (conc.)
• Wash Buffer 2 (conc.)
• Elution Buffer