MagJET Genomic DNA Kit
Purifies genomic DNA from cell culture, tissue samples, bacteria, and yeast with a magnetic bead separation method amenable to high-throughput workflows.
Detailní popis
The Thermo Scientific MagJET Genomic DNA Kit is designed for fast and efficient purification of genomic DNA from tissue and cell cultures, bacteria and yeast, as well as from human body samples, such as buccal and urogenital swabs, urine, saliva and hair follicles.
The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET magnetic beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual purification by low sample throughput users.
The resulting high quality DNA is free of proteins, nucleases and other contaminants or inhibitors, can be used in a wide range of downstream applications such as PCR, qPCR and other enzymatic reactions. See Table 1 for typical genomic DNA yields from various sources.
Highlights
- Versatile – suitable for gDNA purification from variety of biological samples, including gDNA from eukaryotic cells, animal tissues, yeast and bacteria, as well as from human body samples, such as buccal and urogenital swabs, urine, saliva and hair follicles
- Efficient – high yields of high molecular weight genomic DNA
- High quality – isolates high integrity gDNA, free of inhibitors and RNA contamination
- Flexible – easily adjustable for high throughput automation platforms and manual processing
Applications
Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
- PCR and qPCR
- FastDigest and conventional restriction digestion
- Southern blotting
Includes
- Digestion Buffer
- Lysis Buffer
- MagJET Magnetic Beads
- RNase A
- Proteinase K
- Wash Buffer 1 (conc.)
- Wash Buffer 2 (conc.)
- Elution Buffer
| Source | Quantity | DNA yield | |
|---|---|---|---|
| 1 | Mouse heart | 5 mg | 4.5 µg |
| 20 mg | 18-19 µg | ||
| 2 | Mouse tail (homogenized) | 5 mg | 6 µg |
| 20 mg | 15 µg | ||
| 3 | Mouse tail (cut) | 5 mg | 1 µg |
| 20 mg | 4-5 µg | ||
| 4 | Mouse liver | 5 mg | 14-15 µg |
| 5 | Mouse spleen | 5 mg | 20 µg |
| 6 | Mouse kidney | 5 mg | 9-10 µg |
| 20 mg | 35-38 µg | ||
| 7 | Mouse muscle | 5 mg | 2.5 µg |
| 20 mg | 8-9 µg | ||
| 8 | Mouse brain | 5 mg | 3 µg |
| 20 mg | 10.5-11 µg | ||
| 9 | Mouse skin | 5 mg | 5 µg |
| 20 mg | 18-19 µg | ||
| 10 | Mouse lung | 5 mg | 9-10 µg |
| 11 | Mouse ear | 5 mg | 9.5 -10 µg |
| 20 mg | 35-37 µg | ||
| 12 | E. coli cells | ~2 × 109 cells | 13-17 µg |
| 13 | S. cerevisiae | ~ 108 cells | 7-11 µg |
| 14 | HeLa cells | ~ 106 cells | 7-11 µg |
| 15 | Jurkat cells | ~ 106 cells | 6-12 µg |
| 16 | COS-7 cells | ~ 106 cells | 3-5 µg |
| 17 | Human blood leucocytes | leucocytes collected from 1 mL of blood | 13-17 µg |
| CategoryName | |
| Hazardous | Yes |
| Hazardous: | No |
| Quality Control | The MagJET Genomic DNA Kit is qualified by isolating genomic DNA from 5 mg of mouse liver following the protocols outlined in the manual. The quality of purified genomic DNA is evaluated spectrophotometrically and by agarose gel electrophoresis. The purified genomic DNA has an A260/A280 ratio of 1.8±0.2. The functional quality of purified DNA is evaluated by digestion with restriction endonucleases. |
| Shelf Life | 36 Months |
| Shelf Life: | |
| Shipping Condition | Ambient |
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| Storage Condition | Ambient |
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