LEXSY BHI - Liquid Media Kit
Detailní popis
recommended for transfection and strain maintenance
For general laboratory use. Not intended for human or animal diagnostic or therapeutic uses.
Shipping: Shipped at ambient temperature/on gel packs
Storage Conditions: Store components as indicated on individual labels
Shelf Life: 12 months
Applications:
For the growth of LEXSY hosts P10 (Cat.-No. LT-101) and T7-TR (Cat.-No. LT-110).
Description:
Three-component Kit for preparation of complex Leishmania cultivation medium.
Content:
LEXSY BHI, liquid
Hemin stock solution, 500x (Cat.-No. ML-108):
0,25 % solution of Hemin in 30 % Triethanolamine, sterile
Pen-Strep stock solution, 200x (Cat.-No. ML-105):
10.000 U/ml of penicillin G sodium salt and 10.000 μg/ml of streptomycin sulfate in 0.85 % saline, sterile
| Cat. No. | Content | Amount |
| ML-411S | 1 l medium 2 ml Hemin 5 ml Pen-Strep |
for 1 l medium |
| ML-411L | 5 x 1 l medium 10 ml Hemin 25 ml Pen-Strep |
for 5 l medium |
Storage conditions:
LEXSY BHI, liquid:
Store at room temperature
Stable for 12 months
Hemin stock solution, 500x:
Store at 4 °C in the dark
Stable for 12 month
Pen-Strep stock solution, 200x:
Store at -20 °C
Stable for 12 month
Preparation of complete Leishmania cultivation medium:
Add to 1 l LEXSY BHI:
5.0 ml Pen-Strep solution
2.0 ml Hemin stock solution to a final concentration of 5 μg/ml
Final pH = 7.6 ± 0.2
Store in the dark at 4 °C.
Completed medium is useable for up to 2 weeks. If the completed medium is to be used after this period, appropriate amounts of Hemin and Pen-Strep have to be re-added.
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