LE Agarose
Detailní popis
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 24 months
Form: fine homogeneous powder
Color: white
Applications:
- Nucleic acid analytical and preparative electrophoresis
- Blotting
- Protein electrophoresis such as radial immunodiffusion
Description:
LE Agarose is a general use agarose for routine nucleic acid analytical and preparative electrophoresis of fragments between 200 - 20000 bp, blotting and protein electrophoresis. This agarose has a low electroendosmosis value (EEO), ensuring high electrophoretic mobility of nucleic acids. Because of the high gel strength, easy-to-handle gels can be prepared with this agarose at concentrations as low as 0.4 %. LE Agarose has an exceptionally low fluorescence background.
Specifications:
| Moisture | ≤ 10 % |
| Ash | ≤ 0.4 % |
| Electroendosmosis | 0.05 - 0.13 |
| Sulphate | ≤ 0.1 % |
| Clarity 1.5 % (NTU) | ≤ 3 |
| Gel Strength 1 % | ≥ 1200 g/cm2 |
| Gel Strength 1.5 % | ≥ 2500 g/cm2 |
| Gelling Temperature 1.5 % | 36 ± 1.5 °C |
| Melting Temperature 1.5 % | 88 ± 1.5 °C |
Functional tests
| DNase/RNase activity | none detected |
| DNA resolution > 1000 bp | finaly resolved |
| Gel background | very low |
Recommended Agarose Concentrations:
| Size Range (base pairs) |
Final Agarose Concentration [%] 1x TAE Buffer |
| 1000 - 20000 | 0.60 |
| 500 - 12000 | 0.80 |
| 300 - 8000 | 1.00 |
| 200 - 6000 | 1.20 |
| 100 - 3000 | 1.50 |
| 100 - 2000 | 2.00 |
BIOZ Product Citations:
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