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Lambda DNA/HindIII Marker

Kód produktu: SM0101 Kód výrobce: SM0101 Kód dodavatele: {9B77EB06-9E99-4086-8826-1F15892B8A4C} Výrobce: Life Technologies Czech Republic s.r.o.
2 134,44 Kč
1 764,00 Kč bez DPH
do týdne

A conventional marker composed of 8 sharp bands designed for sizing and approximate quantification of large double-stranded DNA fragments in agarose gels. .

Zobraz detailní popis
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Detailní popis

The Thermo ScientitficLambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientitfic restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

The marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction (see Resources for Protocols).

Highlights

  • Sizing and approximate quantification of large DNA fragments
  • Sharp bands
  • Supplied with loading dye for sample DNA

Note

The cohesive ends (the 12nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5minutes and then cooling on ice for 3minutes.

6X DNA Loading Dye 10mM Tris-HCl (pH7.6), 0.03%bromophenol blue, 0.03%xylene cyanol FF, 60%glycerol, and 60mM EDTA.
Hazardous No
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The DNA concentration is determined spectrophotometrically.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Buffer (TE buffer) 10mM Tris-HCl (pH7.6) and 1mM EDTA.
Storage Condition -20 C

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