Lambda DNA/HindIII Marker
A conventional marker composed of 8 sharp bands designed for sizing and approximate quantification of large double-stranded DNA fragments in agarose gels. .
Detailní popis
The Thermo ScientitficLambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientitfic restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.
The marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction (see Resources for Protocols).
Highlights
- Sizing and approximate quantification of large DNA fragments
- Sharp bands
- Supplied with loading dye for sample DNA
Note
The cohesive ends (the 12nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5minutes and then cooling on ice for 3minutes.
| 6X DNA Loading Dye | 10mM Tris-HCl (pH7.6), 0.03%bromophenol blue, 0.03%xylene cyanol FF, 60%glycerol, and 60mM EDTA. |
| Hazardous | No |
| Quality Control |
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| Storage Buffer (TE buffer) | 10mM Tris-HCl (pH7.6) and 1mM EDTA. |
| Storage Condition | -20 C |
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