Tento e-shop využívá cookies

Na našich webových stránkách používáme soubory cookies. Některé z nich jsou nezbytné, zatímco jiné nám pomáhají vylepšit tento web a váš uživatelský zážitek. Souhlasíte s používáním všech cookies?

Cookies nastavení

Vaše soukromí je důležité. Můžete si vybrat nastavení cookies níže.

Lambda DNA/EcoRI Marker

Kód produktu: SM0281 Kód výrobce: SM0281 Kód dodavatele: {0CE2AEE2-247E-4CE3-9D3F-9599A8AFBFAC} Výrobce: Life Technologies Czech Republic s.r.o.
2 049,74 Kč
1 694,00 Kč bez DPH
do týdne

Lambda DNA/EcoRI Marker is a conventional marker for sizing and approximate quantification of large double-stranded DNA fragments in agarose gels. .

Zobraz detailní popis
  • Zeptejte se odborníka

Detailní popis

Thermo Scientifici Lambda DNA is digested to completion with the appropriate Thermo Scientitific restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

The Thermo Scientific marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction (see Recources for Protocols).

Highlights

  • Sizing and approximate quantification of large DNA fragments
  • Sharp bands
  • Supplied with loading dye for sample DNA

Note

The cohesive ends (the 12nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5minutes and then cooling on ice for 3minutes.

6X DNA Loading Dye 10mM Tris-HCl (pH7.6), 0.03%bromophenol blue, 0.03%xylene cyanol FF, 60%glycerol, and 60mM EDTA.
Hazardous No
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The DNA concentration is determined spectrophotometrically.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Buffer (TE buffer) 10mM Tris-HCl (pH7.6) and 1mM EDTA.
Storage Condition -20 C

Hodnocení produktu

Produkt zatím nikdo nehodnotil, buďte první!