Thermo Scientifici Lambda DNA is digested to completion with the appropriate Thermo Scientitific restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.
The Thermo Scientific marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction (see Recources for Protocols).
- Sizing and approximate quantification of large DNA fragments
- Sharp bands
- Supplied with loading dye for sample DNA
The cohesive ends (the 12nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5minutes and then cooling on ice for 3minutes.
|6X DNA Loading Dye||10mM Tris-HCl (pH7.6), 0.03%bromophenol blue, 0.03%xylene cyanol FF, 60%glycerol, and 60mM EDTA.|
|Storage Buffer (TE buffer)||10mM Tris-HCl (pH7.6) and 1mM EDTA.|
|Storage Condition||-20 C|
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