Labeling Buffer
Detailní popis
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C or -20 °C
Shelf Life: 12 months
Form: liquid
Concentration: 10x conc.
Description:
Labeling Buffer is recommended for DNA labeling or mutagenesis. The PCR buffer system is specially optimized for incorporation of labeled or modified nucleotides into DNA. It gives superior results in a broad range of reaction conditions with most primer-template pairs but amplification may also tend to an increased unspecifity.
Content:
Labeling Buffer (green cap)
600 mM Tris-HCl (pH 8.8, 25 °C), 150 mM (NH4)2SO4, 20 mM MgCl2, 0.05 % Tween-20 and 0.05 % Nonidet P-40
Optimization of MgCl2 concentration:
A final Mg2+ concentration of 2.0 mM is recommended for most DNA labeling applications. However, if an individual Mg2+ optimization is essential add 25 mM MgCl2 stock solution (#PCR-266) as shown in the table below.
| final MgCl2 conc. | 20 μl final assay volume | 50 μl final assay volume |
| 2 mM | - | - |
| 3 mM | 0.8 μl | 2.0 μl |
| 4 mM | 1.6 μl | 4.0 μl |
| 5 mM | 2.4 μl | 6.0 μl |
Related products: Mg2+ Stock, #PCR-266
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