KCl Buffer
Detailní popis
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C or -20 °C
Shelf Life: 12 months
Form: Liquid
Concentration: 10x conc.
Description:
PCR Buffer KCl is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by-product formation.
Content:
PCR Buffer KCl (green cap)
200 mM Tris-HCl (pH 8.5, 25 °C), 500 mM KCl and 15 mM MgCl2.
Optimization of MgCl2 concentration:
A final Mg2+ concentration of 1.5 mM is recommended for most PCR applications if using PCR Buffer KCl. For an individual optimization of the reaction buffer add 25 mM MgCl2 stock solution (PCR-266) as shown in the table below.
| final MgCl2 conc. | 20 μl final assay volume | 50 μl final assay volume |
| 1.5 mM | - | - |
| 2 mM | 0.4 μl | 1.0 μl |
| 3 mM | 1.2 μl | 3.0 μl |
| 4 mM | 2.0 μl | 5.0 μl |
| 5 mM | 2.8 μl | 7.0 μl |
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
Hodnocení produktu
Produkt zatím nikdo nehodnotil, buďte první!