IPTG Solution, ready-to-use

Kód produktu: R1171 Kód výrobce: R1171 Kód dodavatele: {5CE15FB1-F547-4D22-B92B-A2FA3516FA10} Výrobce: UAB Fermentas
2 182,84 Kč
1 804,00 Kč bez DPH
do týdne
10x1,5 ml

Formula: C9H18O5S, Molecular weight: 238.3

IPTG induces the expression of genes under the control of lac operon and is suitable for cloning and protein expression procedures.
Zobraz detailní popis

Detailní popis

Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a  highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon (see Reference 1). It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.

Applications

  • Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (see Reference 1)
  • Induction of gene expression under control of the lac promoter
IPTG Chemical Structure

IPTG
Formula:
C9H18O5S
Molecular weight: 238.3

Note

  • Preparation of a 100 mM (23.83 mg/mL) stock solution in water is recommended
  • For blue/white colony screening use 0.1 mM final IPTG concentration in LB (Luria Broth) media
  • Store IPTG Solution at -20°C. IPTG, dioxane-free, can be stored at +4°C
Hazardous No
Quality Control
  • Functionally tested in blue/white cloning assay
  • Purity of ≥ 99.0% confirmed by HPLC
Storage Condition 4 C,-20 C

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