IPTG, dioxane-free
Kód produktu: R0391
Kód výrobce: R0391
Kód dodavatele: {81C4DB27-60AE-46B6-812C-0675D585B223}
Výrobce:
ThermoFisher Scientific
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Formula: C9H18O5S, Molecular weight: 238.3
IPTG induces the expression of genes under the control of lac operon and is suitable for cloning and protein expression procedures.
Detailní popis
Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon (see Reference 1). It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.
Applications
- Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (see Reference 1)
- Induction of gene expression under control of the lac promoter
IPTG
Formula: C9H18O5S
Molecular weight: 238.3
Note
- Preparation of a 100 mM (23.83 mg/mL) stock solution in water is recommended
- For blue/white colony screening use 0.1 mM final IPTG concentration in LB (Luria Broth) media
- Store IPTG Solution at -20°C. IPTG, dioxane-free, can be stored at +4°C
| Hazardous | No |
| Quality Control |
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| Storage Condition | 4 C,-20 C |
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