HighPrep Viral DNA/RNA Kit (384 preps)
Magnetic beads based kit for rapid isolation of total viral nucleic acids from whole blood, serum, plasma, saliva and other body fluids. OPTIMIZED FOR ISOLATION OF COVID-19 RNA
Detailní popis
Viral DNA and RNA isolation for:
- RT-qPCR; RT-PCR
- Virus detection, genotyping
- Viral load monitoring
- OPTIMIZED FOR ISOLATION OF COVID-19 RNA
- Rapid and reliable purification of viral nucleic acids
- Adaptable to various automated liquid handling workstations
- No toxic organic solvents
The HighPrep™ Viral DNA/RNA kit is designed for rapid and reliable isolation of total viral nucleic acids from whole blood, serum, plasma, saliva and other body fluids. High-quality DNA is suitable for direct use in most downstream applications such as nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows as well as adapted to major liquid handling workstation in the market.
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
- Prepare lysis mastermix using VDR Lysis Buffer, Carrier RNA and Isopropanol
- Add plasma to the mix
- Add MAG-S1 particles and Pro K Solution
- Add HSW Buffer
- Wash 2 times with 70% ethanol
- Elution done using nuclease free water
- Store NA at -20°C
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