GeneRuler™ Express DNA Ladder, ready-to-use
Detailní popis
Thermo Scientific GeneRuler Express DNA Ladder, ready-to-use, is designed for fast separation (in 5 to 15 minutes at 23 V/cm) of double-stranded DNA fragments in agarose gels (see Figure 1 and Table 1). The band pattern of express ladders is not affected by the composition of electrophoresis buffer, voltage, or gel percentage. The ladder is a mixture of chromatography-purified individual DNA fragments.
GeneRuler Express DNA Ladder, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading onto agarose gels.
The ladder is supplied with 6X DNA Loading Dye for sample DNA.
Highlights
- Ideal for both DNA sizing and approximate quantification
- Ideal for fast separation under a wide range of electrophoresis conditions
- Sharp bands
- Bright reference bands (given in red)
- Can be directly loaded and are stable at room temperature for 6 months
- Supplied with loading dye for sample DNA
6X DNA Loading Dye | 10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA. |
GeneRuler Storage and Loading Buffer (for ready-to-use ladders) | 10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF, and 10% glycerol. |
Hazardous | No |
Quality Control |
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Storage Condition | -20 C |
Storage Notes | Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C. |
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