GeneJET Stab. /Fresh Blood RNA

Detailní popis

The GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).

Highlights

• Versatile – compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus ™ Tubes
• Convenient – specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h
• Efficient – up to 4 µg of total RNA from 500 µL of healthy human blood
• Pure – isolates intact total RNA free of any RT-qPCR inhibitors

Applications

Isolated total RNA can be used in:

• RT-PCR
• RT-qPCR
• Northern Blotting

Includes

• Lysis Buffer
• Wash Buffer 1 (conc.)
• Wash Buffer 2 (conc.)
• DNase l (lyophilized)
• DNase I Reconstitution Buffer
• 2x DNase I Buffer
• Manganese Chloride Solution
• Water, nuclease-free
• GeneJET RNA Purification Columns pre-assembled with Collection Tubes
• Collection Tubes, 2 mL
• Collection Tubes, 1.5 mL