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GeneJET™ RNA Purification Kit

Kód produktu: K0732 Kód výrobce: K0732 Kód dodavatele: {F42FBBB3-DDF7-46B3-B4AD-909168F0214D} Výrobce: Life Technologies Czech Republic s.r.o.
35 646,60 Kč
29 460,00 Kč bez DPH
do týdne
250 prep
Silica membrane-based total RNA purification from mammalian blood, cell cultures, and tissues as well as insect, yeast, and bacteria. Provided in a convenient spin-column format.
Zobraz detailní popis

Detailní popis

The Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights

  • Universal – can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
  • Efficient – high yields of total RNA.
  • Fast – 15 minutes after lysis step
  • Pure – an A260/280 ratio > 1.9
  • Convenient – spin columns are capped and assembled with collection tubes

Applications

  • Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
    • RT-PCR
    • RT-qPCR
    • Northern blotting
    • Nuclease protection assay
  • Proteinase K 
  • Lysis Buffer
  • Wash Buffer 1 (concentrated)
  • Wash Buffer 2 (concentrated)
  • Water, nuclease-free .
  • GeneJET RNA Purification Columns (pre-assembled with Collection Tubes)
  • Collection Tubes (2 ml)
  • Collection Tubes (1.5 ml)
  • Detailed Protocol
Table 1. | Typical RNA yields from various sources.
Source Quantity Yield, µg
Mouse heart 20 mg 10 to 15
Mouse muscle 30 mg 8 to 10
Mouse lung 30 mg 25 to 30
Mouse kidney 30 mg 25 to 30
Mouse liver 30 mg 60 to 65
Mouse spleen 5 mg 10 to 15
Bacillus pumilis cells 1 x 109 cells 15 to 20
Escherichia coli cells 1 x 109 cells 25 to 30
HeLa cells 5 x 106 cells 35 to 40
Jurkat cells 5 x 106 cells 40 to 50
Cos-7 cells 1 x 106 cells 20 to 25
Saccharomyces cerevisiae cells 4 x 108 cells 150 to 160
Hazardous No
Quality Control
  • The GeneJET RNA Purification Kit is qualified by isolating total RNA from 20 mg of mouse muscle or heart following the described protocol.
  • The purified RNA has an A260/280 ratio of ≥ 1.9.
  • The RNA integrity numbers (RIN) of RNA purified from mouse muscle and heart are > 7 and > 8, respectively.
  • Functional quality of the purified RNA is evaluated by RT-PCR of mouse dystrophin RNA resulting in a 13.3 kb PCR product.
Storage Condition Ambient

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