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GeneJET™ Plant Genomic DNA Purification Mini Kit

Kód produktu: K0791 Kód výrobce: K0791 Kód dodavatele: {0D1ECED7-3A0A-4682-AEDE-1229AEC52AF0} Výrobce: Life Technologies Czech Republic s.r.o.
5 045,70 Kč
4 170,00 Kč bez DPH
do týdne
50 prep

.Silica membrane-based genomic DNA isolation from a wide variety of plant species and tissue types, including leaves, seeds, and biogen1czs. Provided in a convenient spin column format.

Zobraz detailní popis
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Detailní popis

Thermo Scientific GeneJET Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.


  • Fast – procedure completed in 30 min following cell lysis
  • Convenient – protocol does not include clogging-prone lysate filtration step
  • High yield – up to 32 µg of high quality DNA from 100 mg sample, including nuclear, mitochondrial, and chloroplast DNA
  • Compatible – use with awide variety of samples (leaves, biogen1czs, sprouts and other parts of various plants)

The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (e. g. young wheat leaves) is 30 to 32 μg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, southern blotting, and enzymatic reactions.


  • Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
    • PCR and qPCR
    • FastDigest and conventional restriction digestion
    • Southern blotting


  • RNase A
  • Lysis Buffer A
  • Lysis Buffer B
  • Precipitation Solution
  • Plant gDNA Binding Solution
  • Wash Buffer I (concentrated)
  • Wash Buffer II (concentrated)
  • Elution Buffer
  • GeneJET Genomic DNA Purification Columns (pre-assembled with collection tubes)
  • Collection tubes
  • Detailed Protocol
Hazardous No
Quality Control
  • GeneJET Plant Genomic DNA Purification Mini Kit is qualified by isolating genomic DNA from 100 mg of plant tissue following the protocols outlined in the manual.
  • The purified genomic DNA has an A260/280 ratio between 1.7 and 1.9.
  • A single band of more than 30 kb is observed after agarose gel electrophoresis and ethidium bromide staining.
  • The functional quality of purified genomic DNA is evaluated by digestion with restriction enzymes.
Storage Condition Ambient

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