Fluorescent 20 bp DNA Ladder

Detailní popis

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
store dark
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 10 % (w/v) Glycerol, SYBR® Green and Orange G

Color: orange

Concentration: 105 ng/μl

Description:
Fluorescent 20 bp DNA Ladder consists of 10 DNA fragments ranging from 20 bp to 200 bp. The prestained ladder is supplied in a ready-to-use format containing SYBR® Green fluorescent DNA stain and an orange tracking dye. It allows sizing and concentration estimate of DNA fragments on agarose gels generated by PCR or restriction digest.
DNA log scale ladders are well suited for combination if small and large fragments should be analyzed on one gel.

Fluorescent DNA Stain:
DNA Ladders with Fluorescent Stain contain SYBR® Green a fluorescent DNA intercalator dye specially developed for DNA analysis applications. High quantum yield and excellent stability makes SYBR® Green the ideal fluorophore for DNA staining applications and a superior replacement for Ethidium Bromide.
DNA Ladders with Fluorescent Stain are optimized for direct loading into unstained agarose gels and are recommended for use in combination with Gel Loading Buffer with DNA Stain (#PCR-274 - #PCR-276).
The ladders provide highest convenience during routine handling and avoid commonly used gel stains like Ethidium Bromide.

Recommended Load:
5 μl per lane

DNA Fragments:
20, 40, 60, 80, 100, 120, 140, 160, 180, 200 bp

BIOZ Product Citations:
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