Fluorescein-12-dUTP, 1 mM Solution

Detailní popis

Thermo Scientific Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution. Fluorescein-12-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment and DNA Polymerase I.

Applications

• Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.

  General Characteristics	Exmax = 495 nm; Emmax = 520 nm (pH 9.0), lambdamax = 495 nm, extinction coefficient = 70.0 x 103 M-1 x cm-1 (pH 9.0).
  Hazardous	No
  Quality Control	Functionally tested in cDNA synthesis Greater than 90% purity confirmed by HPLC Free of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities
  Storage Condition	-20 C
  Storage Note	Store at -20°C in the dark.