First Strand cDNA Synthesis Kit

Detailní popis

Thermo Scientific First Strand cDNA Synthesis kit utilizes the recombinant M-MuLV Reverse Transcriptase which exhibits lower RNase H activity than AMV reverse transcriptase. Due to this feature, full-length cDNA can be synthesized from RNA templates up to 9 kb. The recombinant RiboLock RNase Inhibitor, supplied with the kit effectively protects RNA template from degradation.

The first strand of cDNA can be directly used as a template in PCR or in second strand cDNA synthesis.

Highlights

• Efficient synthesis of first strand cDNA up to 9 kb
• Reaction temperature 37°C
• Supplied with the recombinant RiboLock RNase Inhibitor
• Complete – oligo(dT)18 and random hexamer primers included with the kit

Applications

• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Construction of cDNA libraries
• Generation of probes for hybridization
• aRNA synthesis

Includes

• M-MuLV Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix, 10 mM each
• Oligo(dT)₁₈ Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer, 10 µM (5'-CAAGGTCATCCATGACAACTTTG-3')
• Reverse GAPDH Primer, 10 µM (5'-GTCCACCACCCTGTTGCTGTAG-3')
• Water, nuclease free

The kit is supplied with both oligo(dT)₁₈ and random hexamer primers. The oligo(dT)₁₈ anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail e.g. micro RNAs. Gene-specific primers may also be used with the kits.