FastRuler™ Ultra Low Range DNA Ladder, ready-to-use
Detailní popis
FastRuler Ultra Low Range DNA Ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.
The ladder is especially useful for electrophoresis of PCR products.
FastRuler Ultra Low Range DNA Ladder, ready-to-use contains dephosphorylated DNA fragments and is ideal for forward 5-end labeling with T4 Polynucleotide Kinase (see the Certificate of Analysis for the detailed labeling protocol).
Highlights
- Fast separation (8 to 14 min)
- Short separation distance (10 to 20 mm)
- Sharp bands, easy-to-remember fragment sizes and quantities
- Ready-to-use – premixed with loading dye
- Supplied with loading dye solution for sample DNA
6× Orange DNA Loading Dye | 10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA. |
Bands |
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Hazardous | No |
Quality Control |
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Storage | Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C. |
Storage and Loading Buffer | 10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, 10% glycerol. |
Storage Condition | -20 C |
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