Exo-Resistant Random Primer

Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. This primer has two 3'-terminal phosphorothioate (PTO) modifications that are resistant to the 3'→5' exonuclease activity of proofreading DNA polymerases (see Reference 1), such as Klenow Fragment and phi29 DNA Polymerase. It also has 5'- and 3'-hydroxyl ends. The Exo-Resistant Random Primer is supplied as a ready-to-use, 20X concentrated aqueous solution.

Highlights

• 3’-terminal PTO modifications protect from 3’→5’ exonuclease degradation and ensure efficient priming of DNA synthesis

Applications

• Strand displacement amplification of genomic DNA (see​ Reference 2), plasmids and phage DNA (see Reference 3)
• DNA labeling by random priming (see​ References 4-6)

Note

Use of this enzyme in certain applications may be covered by patents and may require a license.