EpiJET 5-hmC Analysis Kit
EpiJET 5-hmC Analysis Kit is used for 5-hmC analysis and quantification in CCGG sequences.
The Thermo Scientific EpiJET 5-hmC Analysis Kit is an efficient system for detection of 5-hydroxymethylcytosine (5-hmC) within specific DNA loci. The kit takes advantage of the T4 phage β-glucosyltransferase (T4 BGT) ability to specifically modify 5-hmC residues by adding glucose moiety to 5-hmC and the sensitivity of restriction enzyme MspI to glucosylated 5-hmC.
The EpiJET 5-hmC Analysis Kit includes two advanced enzymes: T4 BGT and Epi MspI. The T4 BGT is formulated for highly specific, complete, and fast glucosylation of 5-hmC in 15 min. The Epi MspI is an MspI restriction enzyme specially formulated for efficient use in epigenetic studies. It completely digests genomic DNA in 4 hours when cytosine, 5-methylcytosine, or 5-hydroxymethylcytosine is within its recognition sequence, but its activity is completely blocked when 5-hmC is glucosylated. The analysis involves DNA sample glucosylation and subsequent Epi MspI digestion. Restriction digestion products are then analyzed by qPCR with a primer pair flanking
CCGG site of interest (see Figure 1 in Supporting Data). Use the specially designed calculator for calculation of 5-hmC percentage.
- Complete and specific 5-hmC conversion to glucosylated 5-hmC in 15 min
- Discrimination of 5-hmC in
CCGGsequences by Epi MspI digestion in 4 hours
- Quantification of 5-hmC percentage in
CCGGsequences by qPCR
- 5-hmC analysis and quantification in
- T4 β – glucosyltransferase, 5 U/µL
- 10X Epi Buffer
- 10X UDP–glucose
- Epi MspI
- 5-hmC Control DNA
- Control primer 1
- Control primer 2
- Water, nuclease-free .
- Detailed Protocol
|REviewer||Use for DNA sequence analysis and map creation.|
|Storage Condition||-20 C|
Produkt zatím nikdo nehodnotil, buďte první!