Endonuclease IV, E.coli

Kód produktu: EN0591 Kód výrobce: EN0591 Kód dodavatele: {5881F660-ABF0-49EA-B148-634004B8878A} Výrobce: UAB Fermentas
5 432,90 Kč
4 490,00 Kč bez DPH
Skladem
100 Units
Enzyme acting on oxidative damaged DNA which recognizes apurinic/apyrimidinic (AP) sites of dsDNA and cleaves the phosphodiester bond 5' to the lesion generating a hydroxyl group at the 3'-terminus.
Zobraz detailní popis

Detailní popis

Thermo Scientific Endonuclease IV (Endo IV) recognizes apurinic/apyrimidinic (AP) sites of dsDNA and cleaves the phosphodiester bond 5' to the lesion generating a hydroxyl group at the 3'-terminus (see Figure 1 in Supporting Data). The enzyme can also act as a  3'-diesterase that is able to release 3'-phosphoglycolate or 3'-phosphate from the damaged ends of dsDNA (see Reference 1). Endo IV possesses also a 3'=>5' exonuclease activity. Its progression on substrates is sensitive to ionic strength, metal ions, EDTA, and reducing conditions. Substrates with 3'-recessed ends are preferred substrates for the 3'=>5' exonuclease activity (see​ Reference 2).

The enzyme has no requirement for Mg2+ and is fully active in the presence of EDTA in moderate concentrations.

Applications

  • Studies of DNA damage and repair (see​ References 3, 4, 5)
  • Single cell electrophoresis (comet assay) (see​ Reference 6)
  • Antitumor drug research (see​ Reference 4)
  • DNA structure research (see​ References 5, 7)
  • SNP analysis (see​ Reference 8)
10X Reaction Buffer 500 mM Tris-acetate (pH 7.5), 500 mM KCl, 10 mM EDTA, 0.5% (v/v) Triton X-100.
CategoryName
Concentration 2 U/µL
Definition of Activity Unit
  • One unit of the enzyme relaxes 1 µg of partially depurinated, covalently closed supercoiled plasmid DNA in 30 min at 37°C.
  • Enzyme activity is assayed in the following mixture: 50 mM Tris-acetate (pH 7.5), 50 mM KCl, 1 mM EDTA, 0.05% (v/v) Triton X-100, and 2 µg of partially depurinated pUC19 DNA.
Hazardous No
Hazardous: No
Inactivation Inactivated by heating at 80°C for 15 min.
Inhibition Inhibitors: although the enzyme is fairly resistant to EDTA during reaction, it becomes sensitive to even submillimolar quantities of chelators when no DNA substrate is present.
Molecular Weight 31.6 kDa monomer
Quality Control The absence of other endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.
Shelf Life:
Shipping Condition:
Shipping Information
Source E. coli cells with a cloned nfo gene
SpecificationName
SpecificationValue
Storage Buffer The enzyme is supplied in:
50 mM Tris-acetate (pH 7.7), 50 mM KCl, 1 mM DTT, 0.05% (v/v) Triton X-100, and 50% (v/v) glycerol.
Storage Condition -20 C
Storage Condition: -20 C

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