Thermo Scientific Endonuclease IV (Endo IV) recognizes apurinic/apyrimidinic (AP) sites of dsDNA and cleaves the phosphodiester bond 5' to the lesion generating a hydroxyl group at the 3'-terminus (see Figure 1 in Supporting Data). The enzyme can also act as a 3'-diesterase that is able to release 3'-phosphoglycolate or 3'-phosphate from the damaged ends of dsDNA (see Reference 1). Endo IV possesses also a 3'=>5' exonuclease activity. Its progression on substrates is sensitive to ionic strength, metal ions, EDTA, and reducing conditions. Substrates with 3'-recessed ends are preferred substrates for the 3'=>5' exonuclease activity (see Reference 2).
The enzyme has no requirement for Mg2+ and is fully active in the presence of EDTA in moderate concentrations.
- Studies of DNA damage and repair (see References 3, 4, 5)
- Single cell electrophoresis (comet assay) (see Reference 6)
- Antitumor drug research (see Reference 4)
- DNA structure research (see References 5, 7)
- SNP analysis (see Reference 8)
|10X Reaction Buffer||500 mM Tris-acetate (pH 7.5), 500 mM KCl, 10 mM EDTA, 0.5% (v/v) Triton X-100.|
|Definition of Activity Unit||
|Inactivation||Inactivated by heating at 80°C for 15 min.|
|Inhibition||Inhibitors: although the enzyme is fairly resistant to EDTA during reaction, it becomes sensitive to even submillimolar quantities of chelators when no DNA substrate is present.|
|Molecular Weight||31.6 kDa monomer|
|Quality Control||The absence of other endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.|
|Source||E. coli cells with a cloned nfo gene|
|Storage Buffer||The enzyme is supplied in:
50 mM Tris-acetate (pH 7.7), 50 mM KCl, 1 mM DTT, 0.05% (v/v) Triton X-100, and 50% (v/v) glycerol.
|Storage Condition||-20 C|
|Storage Condition:||-20 C|
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