dUTP, 100 mM Solution

Thermo Scientific dUTP (2'-deoxyuridine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. The nucleotide has greater than 99% purity, is free of nuclease activities, human and E. coli DNA. It is designed for many different molecular biology applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free ofhuman and E. coli DNA
• Highly stable
• Application tested in:
  • Long range PCR (40 kb)
  • cDNA synthesis and RT-PCR
  • Real-time PCR
  • Standard PCR
  • High fidelity PCR

Applications

• For use in all molecular biology applications, including PCR, real-time PCR and RT-PCR with Taq DNA Polymerase, cDNA synthesis, and primer extension.

dUTP
Formula: C₉H₁₂N₂O₁₄P₃Na₃
Molecular weight: 534.1 (acid form: 468.1)

Note

dUTP is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.