dUTP - Solution (100mM)

Detailní popis

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C₉H₁₅N₂O₁₄P₃ (free acid)

Molecular Weight: 468.14 g/mol (free acid)

CAS#: 102814-08-4

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution

Concentration: 100 mM - 110 mM

pH: 8.5 ±0.2 (22 °C)

Spectroscopic Properties: λ_max 262 nm, ε 9.8 L mmol^-1 cm^-1 (Tris-HCl pH 7.0)

Description:
dTTP, PCR-grade is supplied as ultrapure aqueous solution (pH 8.5) and suitable for all molecular biology applications including PCR/qPCR, reverse transcription, DNA labeling and DNA sequencing.

Selected References:
Tóth et al. (2007) Kinetic Mechanism of Human dUTPase, an Essential Nucleotide Pyrophosphatase Enzyme. J. Biol. Chem. 282 (46):33572.
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.