dNTP Mix dUTP - Solution

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula:
dATP: C₁₀H₁₆N₅O₁₂P₃ (free acid)
dCTP: C₉H₁₆N₃O₁₃P₃ (free acid)
dGTP: C₁₀H₁₆N₅O₁₃P₃ (free acid)
dUTP: C₉H₁₅N₂O₁₄P₃ (free acid)

Molecular Weight:
dATP: 491.18 g/mol (free acid)
dCTP: 467.15 g/mol (free acid)
dGTP: 507.18 g/mol (free acid)
dUTP: 468.14 g/mol (free acid)

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution

pH: 8.5 ±0.2 (22 °C)

Description:
dNTP Mix incl. dUTP is an mixture of 10 mM ultrapure dATP, dCTP, and dGTP, and 20 mM dUTP supplied as clear aqueous solution (pH 8.5). dUTP can be used in place of dTTP in PCR and RT-PCR protocols to prevent carry-over contaminations from previous amplifications.

Product Citations:
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Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.