dNTP Mix - 10 mM Solution
Detailní popis
![Structural formula of dNTP Mix - 10 mM Solution (Equimolar Mix of 10 mM dATP, dCTP, dGTP and dTTP, 2'-Deoxyadenosine-5'-triphosphate, Sodium salt; 2'-Deoxycytidine-5'-triphosphate, Sodium salt; 2'-Deoxyguanosine-5'-triphosphate, Sodium salt; 2'-Deoxythymidine-5'-triphosphate, Sodium salt)](https://www.jenabioscience.com/images/Structures/NU-1006_mix.png)
![](https://www.jenabioscience.com/images/cd3d498e66/NU-1006_webbild.jpg)
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.
Shelf Life: 12 months
Molecular Formula:
dATP: C10H16N5O12P3 (free acid)
dCTP: C9H16N3O13P3 (free acid)
dGTP: C10H16N5O13P3 (free acid)
dTTP: C10H17N2O14P3 (free acid)
Molecular Weight:
dATP: 491.18 g/mol (free acid)
dCTP: 467.15 g/mol (free acid)
dGTP: 507.18 g/mol (free acid)
dTTP: 482.17 g/mol (free acid)
Purity: ≥ 99 % (HPLC)
Form: clear aqueous solution
pH: 8.5 ±0.2 (22 °C)
Applications:
For standard PCR applications a final concentration of 200 μM each dNTP is recommended.
Description:
dNTP Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP supplied as clear aqueous solution (pH 8.5).
Quality Control Specifications:
Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template or less
RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template or less
Contamination with bacterial or human DNA: not detectable
DNases, RNases, Nicking Activity: not detectable
Proteases: not detectable
Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.
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