dNTP Bundle (4x20µmol)

Detailní popis

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula:
dATP: C₁₀H₁₆N₅O₁₂P₃ (free acid)
dCTP: C₉H₁₆N₃O₁₃P₃ (free acid)
dGTP: C₁₀H₁₆N₅O₁₃P₃ (free acid)
dTTP: C₁₀H₁₇N₂O₁₄P₃ (free acid)

Molecular Weight:
dATP: 491.18 g/mol (free acid)
dCTP: 467.15 g/mol (free acid)
dGTP: 507.18 g/mol (free acid)
dTTP: 482.17 g/mol (free acid)

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution

Concentration: 100 mM - 110 mM

pH: 8.5 ±0.2 (22 °C)

Spectroscopic Properties: dATP: λ_max 259 nm, ε 15.4 L mmol^-1 cm^-1 (Tris-HCl pH 7.0) dCTP: λ_max 271 nm, ε 8.9 L mmol^-1 cm^-1 (Tris-HCl pH 7.0) dGTP: λ_max 252 nm, ε 13.7 L mmol^-1 cm^-1 (Tris-HCl pH 7.0) dTTP: λ_max 262 nm, ε 9.6 L mmol^-1 cm^-1 (Tris-HCl pH 7.0)

Description:
dNTP Bundle contains four separate solutions of ultrapure dATP, dCTP, dGTP and dTTP supplied as clear aqueous solutions (pH 8.5).

Quality Control Specifications:
Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template or less
RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template or less
Contamination with bacterial or human DNA: not detectable
DNases, RNases, Nicking Activity: not detectable
Proteases: not detectable

Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.