?DNA/ HindIII Digest 100 µg

Detailní popis

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid, green, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 8 % (w/v) Glycerol, Tartrazine and Xylene Cyanol FF

Concentration: 200 ng/μl

Description:
The HindIII digest of λ DNA yields the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125.

* The cohesive ends (12b cos site of bacteriophage lambda) of fragments 23130 bp and 4361 bp may be separated by heating to 65 °C for 5 min.

Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH₂O and subsequently heated.

Recommended Load:
5 μl per lane

Preparation:
Lambda DNA (48.502 bp) was completely digested by HindIII, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.