dATP, 100 mM Solution

Detailní popis

Thermo Scientific dATP (2'-deoxyadenosine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. The nucleotide has greater than 99% purity, is free of nuclease activities, human and E. coli DNA. It is designed for many different molecular biology applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA
• Highly stable
• Application tested in:
  • Long range PCR (40 kb)
  • cDNA synthesis and RT-PCR
  • Real-time PCR
  • Standard PCR
  • High fidelity PCR

Applications

• For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

dATP
Formula: C₁₀H₁₃N₅O₁₂P₃Na₃
Molecular weight: 557.2 (acid form: 491.2)

Extinction Coefficient	15.2 x 103 M-1 x cm-1 (pH 7.0).
Formula	C10H13N5O12P3Na3
Hazardous	No
Lambda max	259 nm
Molecular weight	557.2 (acid form: 491.2)
Quality Control	Functionally tested in PCR Greater than 99% purity confirmed by HPLC Free of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities
Storage Condition	-20 C