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Crystal Taq Master (2x)

Kód produktu: PCR-166S Kód výrobce: PCR-166S Výrobce: Jena Bioscience GmbH
3 668,24 Kč
3 031,60 Kč bez DPH
do týdne

Crystal Taq Master (2x)
Master mix for routine PCR
Ready-to-Use Mixes for PCR

Zobraz detailní popis

Detailní popis

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term storage (up to 3 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid

Concentration: 2x conc.

Crystal PCR Master is a 2 x conc. ready-to-use master mix recommended for routine PCR applications (up to 4 kb fragment length), high throughput PCR or genotyping.
It contains all reagents required for PCR (except template and primer) in a well-balanced ratio to ensure high specificity and minimal by-product formation in almost all PCR applications without the need of additional optimization steps.
The mix guarantees robust and reliable amplification results with a minimum of pipetting steps, saves time and reduces the risk of contaminations.
The total PCR assay volume is freely adaptable to individual protocols or the requirements of automated pipetting systems.


Cat.No. Master Mix PCR-grade water Assays x 50 μl
PCR-166S 4 x 1.25 ml 6 ml 200
PCR-166L 20 x 1.25 ml 2 x 12.5 ml 1000
PCR-166XL 100 ml 100 ml 4000



2 x concentrated PCR master mix containing Taq polymerase, nucleotides (dATP, dCTP, dGTP, dTTP), KCl, (NH4)2SO4, MgCl2, density reagent, enhancing and stabilizing additives.

Recommended PCR assay:
Before starting, vortex the master mix thoroughly to assure homogeneity.

component stock. conc. 20 μl
50 μl
final conc.
Crystal PCR Master 2x 10 μl 25μl 1x
Primer Mix
or each primer
10 μM each primer 0.4-0.8 μl 1-2 μl 200-400 nM each primer
Template/ sample DNA   < 10 ng < 20 ng  
PCR-grade water   fill up to 20 μl fill up to 50 μl  



Recommended cycling conditions:
Before cycling, vortex PCR tubes or plates to assure homogeneity and centrifuge briefly to remove bubbles.

95 °C 2 min 1x
95 °C
50 - 68 °C
72 °C
10 - 20 sec
10 - 20 sec
20 sec - 4 min

25 - 30x

1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.

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