ClaSeek NGS Kit Ion Torrent

Detailní popis

Thermo Scientific ClaSeek Library Preparation Kit, Ion Torrent™ compatible, is designed for fast and convenient construction of amplification-free NGS fragment library from DNA sample input as low as 5 ng. The ClaSeek Library Preparation kit utilizes highly efficient NGS-optimized library construction protocol combining end-repair, cleanup and adapter addition into fast and convenient workflow. Enzyme master mixes included in the kit minimize unnecessary pipetting steps and reduce hands-on time, allowing the PCR-free library construction in as little as 60 minutes. The kit is suitable for construction of 100-, 200-, 300- or 400-base read libraries for use on Ion PGM™ and 150- or 200-base read libraries for use on Ion Proton™ System.

Highlights

• Flexible – universal protocol for variable DNA sample input (5 ng - 1 µg) and different DNA fragment lengths.
• PCR-free – significantly minimized bias and improved coverage with amplification-free protocol
• Fast and Convenient – generates PCR-free library in less than 60 minutes with significantly less hands-on time.

Applications

DNA fragment library preparation for downstream use in next generation sequencing workflow.

Includes

• ClaSeek End Conversion Master Mix, IT
• ClaSeek Ligation Mix, IT
• Adapters, IT
• Library Amplification Primer Mix
• Water, nuclease-free
• Cleanup Columns (pre-assembled with collection tubes)