Buffer Set for Restriction Enzymes

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Our Five Buffer System ensures the optimum reaction conditions for each restriction enzyme. This system consists of 10X B (blue), G (green), O  (orange), R (red), and Tango (yellow) buffers. All restriction enzymes are supplied in color-coded tubes to indicate the recommended reaction buffer. The recommended buffer and/or the universal Tango buffer are supplied with each enzyme.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation. The buffer set for restriction endonucleases contains 1 mL of each B, G, O, R, and Tango buffers. It is supplied in 10X concentrated stocks.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer. However, some enzymes require additives to achieve 100% activity. For example, AjuI, AlfI, BdaI, BplI, BseMII, FaqI, Eco57I, Eco57MI, Hin4I, and TsoI require S-adenosylmethionine, which is supplied with the enzyme, while AarI and BveI require an oligonucleotide (also supplied with the enzyme). Esp3I requires DTT.

The following enzymes require unique buffers for optimal digestion:

AarI
AjiI
BamHI
BfuI
Bpu10I
BseXI
Bsp143I
Cfr9I
Cfr10I
Eam1105I
Ecl136II
Eco52I
EcoRI
KpnI
Lsp1109I
PacI
PasI
Ppu21I
SacI
ScaI
SdaI
SduI
SgeI
TaqI

Tango buffer has been designed for double digestions of DNA with conventional restriction enzymes. Visit the Thermo Scientific DoubleDigest tool for more information.
1X Buffer B Composition 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 0.1 mg/mL BSA.
1X Buffer G Composition 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl, 0.1 mg/mL BSA.
1X Buffer O Composition 50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl, 0.1 mg/mL BSA.
1X Buffer R Composition 10 mM Tris HCl (pH 8.5 at 37°C), 10 mM MgCl2, 100 mM KCl, 0.1 mg/mL BSA.
1X Buffer Tango Composition 33 mM Tris-acetate (pH 7.5 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate, 0.1 mg/mL BSA.
Hazardous No
Storage Condition -20 C