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BspTI (AflII)

Kód produktu: ER0831 Kód výrobce: ER0831 Kód dodavatele: {4EF7A1D0-35A5-4A71-872C-855253A3A2E3} Výrobce: Life Technologies Czech Republic s.r.o.
1 476,20 Kč
1 220,00 Kč bez DPH
do týdne
1000 units

5'...CT T A A G...3'
3'...G A A T TC...5'

Zobraz detailní popis
  • Zeptejte se odborníka

Detailní popis

Conditions for 100% Activity
  • 1X Buffer O: 50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl, and 0.1 mg/mL BSA
  • Incubate at 37°C
Digestion of Agarose-embedded DNA Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Double Digestion Perform double digestion using DoubleDigest.
Hazardous No
Isoschizomers Search for commercial isoschizomers using REsearch.
Storage Buffer BspTI is supplied in: 10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.
Storage Condition -20 C

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X
G (green)
1X
O (orange)
1X
R (red)
1X
Tango
(yellow)
1X / 2X
O (Orange) 37°C 0-20 0-20 100 20-50 0-20 50-100 2X

Methylation Effects

  • Dam: never overlaps - no effect.
  • Dcm: never overlaps - no effect.
  • CpG: never overlaps - no effect.
  • EcoKI: never overlaps - no effect.
  • EcoBI: never overlaps - no effect.

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
0 0-20 50-100
 
Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
3 2 0 0 0 0
pTZ19R/U pTZ57R pBluescriptIIKS(±) pBluescriptIISK(±) pACYC177 pACYC184
0 0 0 0 0 0

New sites generated by ligation

Newly generated recognition sites resulting from ligation of protruding compatible DNA ends

Recognition Sequence Second Enzyme Enzymes recognizing newly generated recognition sequence
C^TTAAG
  • SmoI (SmlI)* (C^TTAAG)
  • BspTI (AflII)/FastDigest AflII (BspTI)
  • FastDigest MseI (SaqAI)
  • SmoI (SmlI)
  • Tru1I (MseI)/FastDigest Tru1I

Newly generated recognition sites resulting from fill-in of 5'-overhang and self-ligation

Recognition Sequence Newly generated sequence after reaction Enzymes that cleave the newly generated sequence
C^TTAAG CTTAATTAAG
  • PacI/FastDigest PacI (TTAAT^TAA)
  • [FastDigest MseI (SaqAI)]
  • TasI (Tsp509I)/FastDigest Tsp509I (TasI)
  • [Tru1I (MseI)/FastDigest Tru1I]

Hodnocení produktu

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