Bsm DNA Polymerase, Large Fragment
FastDigest buffer for 100% activity G buffer for 100% activity LO certified O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 80°C in 10 min
Thermo ScientificBsm DNA Polymerase, Large Fragment, is a portion of DNA polymerase of Bacillus smithii, which catalyzes 5'=>3' synthesis of DNA and lacks 5'→3' and 3'→5' exonuclease activities. Bsm DNA Polymerase, Large Fragment, has a strong strand displacement activity and is active in a wide range of temperatures from 30°C to 63°C, with an optimum of activity at 60°. It is an enzyme with high functional similarity to Bst DNA Polymerase, Large Fragment, and can replace it in most applications.
Not suitable for use in PCR.
- Thermophilic DNA polymerase with strong strand displacement activity
- Isothermal DNA amplification by the method of:
- Loop-mediated isothermal amplification (LAMP) (see Reference1, 2)
- Whole genome amplification (WGA) (see Reference3)
- Ramification amplification (RAM) (see Reference4)
- Random-primed DNA labeling
- Labeling by fill-in 5'-overhangs of dsDNA
Use of this enzyme in certain applications may be covered by patents and may require a license.
|10X Bsm Buffer||200mM Tris-HCl (pH8.8 at 25°C), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1%(v/v) Tween 20.|
|Definition of Activity Unit||
|Inactivation||Inactivated by heating at 80°C for 10min.|
|Molecular Weight||67kDa monomer|
|Quality Control||The absence of endodeoxyribonucleases, exodeoxyribonucleases, ribonucleases, and E.coli genomic DNA is confirmed by appropriate quality test.|
|Source||E.coli cells with a cloned polA gene from Bacillus smithii.|
|Storage Buffer||The enzyme is supplied in 10mM Tris-HCl (pH7.5), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15%(v/v) Triton X-100, 50%(v/v) glycerol.|
|Storage Condition||-20 C|
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