Bsm DNA Polymerase, Large Fragment

Kód produktu: EP0691 Kód výrobce: EP0691 Kód dodavatele: {D9079BA3-6D6E-4AFD-A863-F4AC060EADEE} Výrobce: UAB Fermentas
1 928,74 Kč
1 594,00 Kč bez DPH
do týdne
1600 units

FastDigest buffer for 100% activity G buffer for 100% activity LO certified O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 80°C in 10 min

DNA polymerase with a high functional similarity to Bst DNA Polymerase and with a strong strand displacement activity.
Zobraz detailní popis

Detailní popis

Thermo ScientificBsm DNA Polymerase, Large Fragment, is a portion of DNA polymerase of Bacillus smithii, which catalyzes 5'=>3' synthesis of DNA and lacks 5'→3' and 3'→5' exonuclease activities. Bsm DNA Polymerase, Large Fragment, has a strong strand displacement activity and is active in a wide range of temperatures from 30°C to 63°C, with an optimum of activity at 60°It is an enzyme with high functional similarity to Bst DNA Polymerase, Large Fragment, and can replace it in most applications.

Not suitable for use in PCR.

Highlights

  • Thermophilic DNA polymerase with strong strand displacement activity

Applications

  • Isothermal DNA amplification by the method of:
    • Loop-mediated isothermal amplification (LAMP) (see Reference1, 2)
    • Whole genome amplification (WGA) (see​ Reference3)
    • Ramification amplification (RAM) (see​ Reference4)
     
  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA

Note

Use of this enzyme in certain applications may be covered by patents and may require a license.

10X Bsm Buffer 200mM Tris-HCl (pH8.8 at 25°C), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1%(v/v) Tween 20.
CategoryName
Definition of Activity Unit

 

  • One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 60°C.
  • Enzyme activity is assayed in the following mixture: 10 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 100 µg/mL BSA, 0.75 mM activated salmon milt DNA, 0.2 mM of each dNTP, 0.4 MBq/mL [H3]-dTTP.
 
Hazardous No
Hazardous: No
Inactivation Inactivated by heating at 80°C for 10min.
Molecular Weight 67kDa monomer
Quality Control The absence of endodeoxyribonucleases, exodeoxyribonucleases, ribonucleases, and E.coli genomic DNA is confirmed by appropriate quality test.
Shelf Life:
Shipping Condition:
Shipping Information
Source E.coli cells with a cloned polA gene from Bacillus smithii.
SpecificationName
SpecificationValue
Storage Buffer The enzyme is supplied in 10mM Tris-HCl (pH7.5), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15%(v/v) Triton X-100, 50%(v/v) glycerol.
Storage Condition -20 C
Storage Condition:

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