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Potvrzuji
  1. Úvodní strana
  2. Reagencie
  3. Life Technologies / Fermentas
  4. Conventional restriction enzymes
  5. Bme1390I (ScrFI)

Bme1390I (ScrFI)

Kód produktu: ER1422 Kód výrobce: ER1422 Kód dodavatele: {4AF59947-583F-40B3-A18B-F448A2ABCB29} Výrobce: Life Technologies Czech Republic s.r.o.
Bme1390I (ScrFI)
5 929,00 Kč
4 900,00 Kč bez DPH
do týdne
2500 Unit

5'...C C↓N G G...3'
3'...G G N↑C C...5'

Zobraz detailní popis

Detailní popis

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

Assayed using lambda DNA (dcm-) (#SD0021). Bme1390I is blocked by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163.

For methylation sensitivity refer to product specifications.

Compatible Ends CC↓SGG - BcnI, SatI
CC↓WGG - MvaI, SatI
Conditions for 100% Activity
  • 1X Buffer O: 50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl, and 0.1 mg/ml BSA
  • Incubate at 37°C
Double Digestion Perform double digestion using DoubleDigest.
Hazardous No
Isoschizomers Search for commercial isoschizomers using REsearch.
Storage Buffer Bme1390I is supplied in: 10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.
Storage Condition -20 C

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X		 G (green)
1X		 O (orange)
1X		 R (red)
1X		 Tango (yellow)
1X / 2X
O (Orange) 37°C 20-50 50-100 100 50-100 50-100 50-100 1X or 2X

Incubation at 30°C results in a 2.5-fold increase in activity.

Methylation Effects

  • Dam: never overlaps - no effect.
  • Dcm: may overlap - blocked.
  • CpG: may overlap - blocked.
  • EcoKI: never overlaps - no effect.
  • EcoBI: never overlaps - no effect.
Methylation type Sequence Cleavage effect
Dcm (CCWGG) 5'...Cm5CW GG...3'
3'...G GWm5CC...5'		 Blocked
CpG 5'...Cm5C GGG...3'
3'...G Gm5CCC...5'		 Blocked

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
20-50 50-100

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
185 3 11 16 12 12
pTZ19R/U pTZ57R pBluescriptIIKS(±) pBluescriptIISK(±) pACYC177 pACYC184
10 10 11 11 14 22

New sites generated by ligation

Newly generated recognition sites resulting from fill-in of 5'-overhang and self-ligation

Recognition Sequence Newly generated sequence after reaction Enzymes that cleave the newly generated sequence
CC^NGG CCNNGG
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
CC^AGG CCAAGG
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
  • Eco130I (StyI)/FastDigest StyI (Eco130I)
CC^CGG CCCCGG
  • [BcnI (NciI)/FastDigest NciI (BcnI)]
  • [Bme1390I (ScrFI)/FastDigest ScrFI (Bme1390I)]
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
  • [BssKI]
  • [HpaII/FastDigest HpaII]
  • [MspI (HpaII)/FastDigest MspI]
CC^GGG CCGGGG
  • [BcnI (NciI)/FastDigest NciI (BcnI)]
  • [Bme1390I (ScrFI)/FastDigest ScrFI (Bme1390I)]
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
  • [BssKI]
  • [HpaII/FastDigest HpaII]
  • [MspI (HpaII)/FastDigest MspI]
CC^TGG CCTTGG
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
  • Eco130I (StyI)/FastDigest StyI (Eco130I)

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