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Biotin Chromogenic Detection Kit

Kód produktu: K0661 Kód výrobce: K0661 Kód dodavatele: {0C975BF7-D892-427A-B163-3030ED9AB04B} Výrobce: Life Technologies Czech Republic s.r.o.
4 501,20 Kč
3 720,00 Kč bez DPH
do týdne
10 rxns

Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. .

Zobraz detailní popis

Detailní popis

The Thermo Scientific Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. The kit is optimized to reproducibly provide high sensitivity with a low background in applications, such as Southern, Northern, dot and slot blotting, and screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods, including the Biotin DecaLabel DNA Labeling Kit.

Features

Biotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP). Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes. The probes are then visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate. Visualization does not require X-ray film or other specific equipment (see Figure 1).

Highlights

  • High sensitivity of detection – 0.03 pg of homologous DNA in dot blot hybridization with biotin-labeled probe
  • Low background due to optimized washing and blocking procedures
  • Non-radioactive detection visualization does not require X-ray film or other specific equipment (see Figure 2)
  • Fast and convenient – ready-to-use components

Applications

  • Southern, Northern, dot/slot blots, plaque, or colony screening.

Includes

  • Streptavidin-AP Conjugate
  • BCIP/NBT Solution
  • Blocking Reagent
  • 10X Blocking/Washing Buffer
  • 10X Detection Buffer
  • Detailed Protocol

Notes

The positively charged SensiBlot Plus Nylon Membrane is a recommended component of this detection system to ensure the highest sensitivity of detection and the lowest background.

CategoryName
Hazardous No
Hazardous: No
Quality Control The kit is functionally tested in dot-blot hybridization with biotin-labeled probe. 0.03 pg of homologous DNA is detected.
Shelf Life:
Shipping Condition:
Storage Condition -20 C
Storage Condition: -20 C

Obsah balení

10 rxns

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