Biotin Chromogenic Detection Kit

Detailní popis

The Thermo Scientific Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. The kit is optimized to reproducibly provide high sensitivity with a low background in applications, such as Southern, Northern, dot and slot blotting, and screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods, including the Biotin DecaLabel DNA Labeling Kit.

Features

Biotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP). Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes. The probes are then visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate. Visualization does not require X-ray film or other specific equipment (see Figure 1).

Highlights

• High sensitivity of detection – 0.03 pg of homologous DNA in dot blot hybridization with biotin-labeled probe
• Low background due to optimized washing and blocking procedures
• Non-radioactive detection visualization does not require X-ray film or other specific equipment (see Figure 2)
• Fast and convenient – ready-to-use components

Applications

• Southern, Northern, dot/slot blots, plaque, or colony screening.

Includes

• Streptavidin-AP Conjugate
• BCIP/NBT Solution
• Blocking Reagent
• 10X Blocking/Washing Buffer
• 10X Detection Buffer
• Detailed Protocol

Notes

The positively charged SensiBlot Plus Nylon Membrane is a recommended component of this detection system to ensure the highest sensitivity of detection and the lowest background.