BCIP-T

Kód produktu: R0821 Kód výrobce: R0821 Kód dodavatele: {9A11F5B1-F236-45C6-8CD0-C553BCC7EF01} Výrobce: UAB Fermentas
2 320,78 Kč
1 918,00 Kč bez DPH
do týdne

Formula: C8H6NO4BrCIP x C7H9N, Molecular weight: 433.6

BCIP-T (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase used in conjunction with the oxidant NBT (nitro blue tetrazolium, #R0841) to enhance blue color development.
Zobraz detailní popis

Detailní popis

Thermo Scientific Alkaline phosphatase catalyses the removal of a  phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan (see Reference 1) (see Scheme below).

Applications

  • Detection of alkaline phosphatase activity in:
    • Southern blotting
    • Western blotting
    • Northern blotting
    • Dot/slot blots
    • Plaque and colony screening
BCIP-T

Glucuronidase cleaves X-Gluc
BCIP-T
Formula:
C8H6NO4BrCIP x C7H9N
Molecular weight: 433.6

Note

Preparation of a 50 mg/mL stock solution in dimethylformamide is recommended.

Dimerization reaction
Formula C8H6NO4BrCIP x C7H9N
Hazardous No
Molecular weight 433.6
Quality Control Less than 3% water content.
Storage Condition -20 C
Storage Note Store at -20°C in the dark.

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