BCIP-T
Kód produktu: R0822
Kód výrobce: R0822
Kód dodavatele: {FA12B633-05D0-46D7-96D9-99185366F4FB}
Výrobce:
UAB Fermentas
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BCIP-T (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase used in conjunction with the oxidant NBT (nitro blue tetrazolium, #R0841) to enhance blue color development.
Detailní popis
Thermo Scientific Alkaline phosphatase catalyses the removal of a phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan (see Reference 1) (see Scheme below).
Applications
- Detection of alkaline phosphatase activity in:
- Southern blotting
- Western blotting
- Northern blotting
- Dot/slot blots
- Plaque and colony screening
Glucuronidase cleaves X-Gluc
BCIP-T
Formula: C8H6NO4BrCIP x C7H9N
Molecular weight: 433.6
Note
Preparation of a 50 mg/mL stock solution in dimethylformamide is recommended.
| Formula | C8H6NO4BrCIP x C7H9N |
| Hazardous | No |
| Molecular weight | 433.6 |
| Quality Control | Less than 3% water content. |
| Storage Condition | -20 C |
| Storage Note | Store at -20°C in the dark. |
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