Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.
Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.
- Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension
Formula C12H17N3O14P3Na3 General Characteristics lambdamax = 240 nm, extinction coefficient = 11.9 x 103 M-1 x cm-1 (pH 7.0)
lambdamax = 290 nm, extinction coefficient = 7.8 x 103 M-1 x cm-1 (pH 7.0)
Hazardous No Molecular weight 589.2 (acid form: 523.2) Quality Control
- Functionally tested in cDNA synthesis with RevertAid H Minus Reverse Transcriptase
- Greater than 96% purity confirmed by HPLC
- Free of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities
Storage Condition -20 C
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