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ALLin™ TaqDNA Polymerase

Kód produktu: PCE0105 Kód výrobce: PCE0105 Kód dodavatele: {09230E76-EE12-483B-BD5C-E8DA53FE2952} Výrobce: highQu GmbH professionally simple
-4 %
Ušetříte
957,72 Kč
25 858,31 Kč
24 900,59 Kč
20 579,00 Kč bez DPH
do týdne
2500

highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in  demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling.

Zobraz detailní popis
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Detailní popis

Applications


  • Routine PCR up to 6 kb

  • PCR of GC rich templates

  • Colony PCR, Fast PCR, TA cloning





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Benefits


  • Higher yields under standard and fast cycling

  • Increased success in amplification of longer (6 kb) and GC rich templates

  • 5X ALLin™ PCR Buffer contains optimal Mg2+ and dNTP concentrations

highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in  demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 104 (a number of correct nucleotides incorporated before the first error). ALLin™ Taq DNA Polymerase produces A-tailed products suitable for ligating into TA cloning vectors. For the maximum convenience the ALLin™ Taq Mastermix, 2X is available.

Important Notes
  • Take typical measures to prevent PCR cross over contamination, keep your bench clean, wear gloves, use sterile tubes and filter pipet tips.
  • Include a no-template control and positive control in parallel.
  • Thaw and keep reagents on ice. Mix well before use.
  • The longer the amplicon, the longer the extension time: 15 sec/kb - <6kb and 1 sec/kb - <1kb. Use >90 sec extension for multiplexing
  • Run an annealing temperature gradient from 55°C to 65°C to choose the best specificity conditions.
  • Do not use fast cycling for multiplexing.
 
Prepare a 50 µl PCR reaction

 

Rev. & For. Primers variable, up to 0.4 µM final each (2 µl of 10 µM each)
cDNA Template  or
gDNA Template
<100 ng   or
5 - 500 ng
5X ALLin™ PCR Buffer 10 μl
Water to 49 μl
ALLin™ Taq DNA Polymerase, 5u/μl 0.25 - 1 µl

 

  • Mix gently, avoid bubbles.
  • Place into the instrument set like:

 

Initial denaturation 1 cycle: 95°C - 1 min
Denaturation 40 cycles: 95°C - 15 sec
Annealing 40 cycles: 55-65°C – 15 sec
Extension 40 cycles: 72°C – 1- 90 sec (15 sec/kb)

 

After the PCR store probes on ice shortly, store at -20°C for long term.

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