5'...↓10(N) G C A (N)6 T G C (N)12↓...3'
3'...↑12(N) C G T (N)6 A C G (N)10↑...5'
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
- Superior quality – stringent quality control and industry leading manufacturing process
- Convenient color-coded Five Buffer System
- Includes universal Tango buffer for double-digestions
- BSA premixed in reaction buffers
- Wide selection of restriction endonuclease specificities
- Molecular cloning
- Restriction site mapping
- Southern Blot
- Restriction fragment length polymorphism (RFLP)
For methylation sensitivity refer to product specifications..
|Conditions for 100% Activity||
|Digestion of Agarose-embedded DNA||Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.|
|Double Digestion||Perform double digestion using DoubleDigest.|
|Isoschizomers||Search for commercial isoschizomers using REsearch.|
|Note||Complete cleavage of some substrates with AlfI is difficult to achieve. AlfI concentration is determined by the maximum cleavage level achieved when no change in the fragmentation pattern is observed with further enzyme increase.|
|Storage Buffer||AlfI is supplied in: 10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA and 50% (v/v) glycerol.|
|Storage Condition||-20 C|
|Recommended buffer for 100% activity||Optimal tempThermo Scientificure||Enzyme activity in Thermo Scientific buffers, %||Tango buffer for double digestion|
1X / 2X
|R (red) +SAM||37°C||0-20 (+SAM)||0-20 (+SAM)||0-20 (+SAM)||100 (+SAM)||0-20 (+SAM)||20-50 (+SAM)||2X (+SAM)|
- Dam: never overlaps - no effect.
- Dcm: never overlaps - no effect.
- CpG: may overlap - no effect.
- EcoKI: never overlaps - no effect.
- EcoBI: never overlaps - no effect.
|Methylation type||Sequence||Cleavage effect|
Cleavage efficiency close to the termini of PCR fragments
|bp from the recognition site to fragment end|
Number of recognition sites in DNA molecules
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