6X DNA Loading Dye & SDS Solution

Detailní popis

Thermo Scientific 6X DNA Loading Dye & SDS Solution is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins. The presence of SDS eliminates DNA-protein interactions and prevents gel-shifts. The high concentration of EDTA protects DNA from degradation by metal-dependent nucleases.

Highlights

• 1% SDS eliminates DNA-protein interactions, prevents appearance of additional bands due to annealing of DNA molecules with cohesive ends
• 100 mM EDTA inhibits metal-dependent nucleases

Applications

• Analysis of DNA samples with high amounts of DNA binding proteins
• Kinetic experiments
• DNA agarose gel analysis after DNA restriction digestions, ligation, or dephosphorylation reactions

  6X Solution Composition	0.03% bromophenol blue 0.03% xylene cyanol FF 60% glycerol 1% SDS 100 mM EDTA (pH 7.6, adjusted with Tris)
  Hazardous	No
  Quality Control	Tested for DNA sample preparation prior to agarose gel electrophoresis. The absence of deoxyribonucleases confirmed by appropriate tests.
  Storage	Store at room temperature or at 4°C up to 12 months. For longer periods, store at -20°C.
  Storage Condition	-20 C