6X DNA Loading Dye & SDS Solution

Kód produktu: R1151 Kód výrobce: R1151 Kód dodavatele: {5DE32AF6-9D8C-42C4-85CB-1EDD28C884F0} Výrobce: UAB Fermentas
1 236,62 Kč
1 022,00 Kč bez DPH
do týdne
5 x 1.0 mL
DNA loading dye containing SDS is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins.
Zobraz detailní popis

Detailní popis

Thermo Scientific 6X DNA Loading Dye & SDS Solution is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins. The presence of SDS eliminates DNA-protein interactions and prevents gel-shifts. The high concentration of EDTA protects DNA from degradation by metal-dependent nucleases.

Highlights

  • 1% SDS eliminates DNA-protein interactions, prevents appearance of additional bands due to annealing of DNA molecules with cohesive ends
  • 100 mM EDTA inhibits metal-dependent nucleases

Applications

  • Analysis of DNA samples with high amounts of DNA binding proteins
  • Kinetic experiments
  • DNA agarose gel analysis after DNA restriction digestions, ligation, or dephosphorylation reactions
    6X Solution Composition
    • 0.03% bromophenol blue
    • 0.03% xylene cyanol FF
    • 60% glycerol
    • 1% SDS
    • 100 mM EDTA (pH 7.6, adjusted with Tris)
    Hazardous No
    Quality Control
    • Tested for DNA sample preparation prior to agarose gel electrophoresis.
    • The absence of deoxyribonucleases confirmed by appropriate tests.
    Storage Store at room temperature or at 4°C up to 12 months. For longer periods, store at -20°C.
    Storage Condition -20 C

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