6X DNA Loading Dye

Detailní popis

Thermo Scientific 6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solution binds divalent metal ions and inhibits metal-dependent nucleases.

6X DNA Loading Dye is used for conventional DNA electrophoresis (see Recommendations for use).

Highlights

• Two-color tracking of DNA migration during DNA electrophoresis
• No DNA masking during gel exposure to UV light
• EDTA binds divalent metal ions and inhibits metal dependent nucleases

Applications

• Preparation of DNA ladders, markers, and samples for loading on agarose or polyacrylamide gels

  Composition	10 mM Tris-HCl (pH 7.6) 0.03% bromophenol blue 0.03% xylene cyanol FF 60% glycerol 60 mM EDTA
  Hazardous	No
  Quality Control	Tested for DNA sample preparation prior to agarose gel electrophoresis. The absence of deoxyribonucleases confirmed by appropriate tests.
  Storage	Store at room temperature or at 4°C up to 12 months. For longer periods, store at -20°C.
  Storage Condition	-20 C