50X TAE Buffer (Tris-acetate-EDTA)

Kód produktu: B49 Kód výrobce: B49 Kód dodavatele: {A6A91F08-5449-416F-9C24-17D82713A10D} Výrobce: UAB Fermentas
2 468,40 Kč
2 040,00 Kč bez DPH
do týdne
1 l
Store at room temperature
50X TAE Buffer (Tris-acetate-EDTA) is used in electrophoresis of nucleic acids in agarose and polyacrylamide gels.
Zobraz detailní popis

Detailní popis

Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.

Applications

  • Electrophoresis of nucleic acids in agarose and polyacrylamide gels
  • Used both as a running buffer and as a gel preparation buffer
  • Filtered through a 0.22 µm membrane
  • Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA

Note

TAE buffer has a relatively low buffering capacity. Therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended.

1X Composition 40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH of 50X TAE: 8.4
Hazardous No
Quality Control The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests.
Storage There are no time limitations for storage of the electrophoresis buffers at room temperature. If the buffer is stored at lower temperatures, a  precipitate may form, which is easily dissolved by gentle heating.
Storage Condition Ambient
Usage Recommendations
  • Use fresh 1X TAE both for the gel and for the electrophoresis run.
  • To prepare 1X TAE buffer add 20 mL of 50X TAE buffer to 980 mL of deionized water and mix well.

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