50X TAE Buffer (Tris-acetate-EDTA)
Kód produktu: B49
Kód dodavatele: {A6A91F08-5449-416F-9C24-17D82713A10D}
Výrobce:
Life Technologies Czech Republic s.r.o.
50X TAE Buffer (Tris-acetate-EDTA) is used in electrophoresis of nucleic acids in agarose and polyacrylamide gels.
Detailní popis
Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.
Applications
- Electrophoresis of nucleic acids in agarose and polyacrylamide gels
- Used both as a running buffer and as a gel preparation buffer
- Filtered through a 0.22 µm membrane
- Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA
Note
TAE buffer has a relatively low buffering capacity. Therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended.
| 1X Composition | 40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH of 50X TAE: 8.4 |
| Hazardous | No |
| Quality Control | The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests. |
| Storage | There are no time limitations for storage of the electrophoresis buffers at room temperature. If the buffer is stored at lower temperatures, a precipitate may form, which is easily dissolved by gentle heating. |
| Storage Condition | Ambient |
| Usage Recommendations |
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