Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.
- Electrophoresis of nucleic acids in agarose and polyacrylamide gels
- Used both as a running buffer and as a gel preparation buffer
- Filtered through a 0.22 µm membrane
- Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA
TAE buffer has a relatively low buffering capacity. Therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended.
|1X Composition||40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH of 50X TAE: 8.4|
|Quality Control||The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests.|
|Storage||There are no time limitations for storage of the electrophoresis buffers at room temperature. If the buffer is stored at lower temperatures, a precipitate may form, which is easily dissolved by gentle heating.|
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