5-Fluoroorotic Acid
Detailní popis
The Thermo Scientific 5-Fluoroorotic Acid is a yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below). Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).
Applications
- Detection of URA3 gene expression in yeast
- Construction of yeast 2-hybrid libraries (see Reference 1)
- Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements, and identification of gene mutations (see Reference 2)
![Convertion of 5-FOA to fluorodeoxyuridine](http://www.thermoscientificbio.com/uploadedImages/Products/Molecular_Labeling_and_Detection/Blotting_and_Detection_Reagents/r081_1.gif)
Convertion of 5-FOA to fluorodeoxyuridine
![5-Fluoroorotic Acid Chemical Structure 5-Fluoroorotic Acid Chemical Structure](http://www.thermoscientificbio.com/uploadedImages/Products/Molecular_Labeling_and_Detection/Blotting_and_Detection_Reagents/r081_fam.gif)
Note
- Low solubility in water
- Solubility in dimethylsulfoxide is greater than 150 mg/mL
- Preparation of a 100 mg/mL solution in dime-thylsulfoxide is recommended
Formula | C5H3FN2O4 x H2O |
Hazardous | No |
Molecular weight | 192.1 |
Quality Control | Purity of 98%. |
Storage | Store at -20°C in the dark. |
Storage Condition | -20 C |
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