5-Fluoroorotic Acid

Kód produktu: R0812 Kód výrobce: R0812 Kód dodavatele: {601E174A-25DE-4E1B-AB62-F93BFDD31E12} Výrobce: UAB Fermentas
9 946,20 Kč
8 220,00 Kč bez DPH
do týdne
5 g
5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase.
Zobraz detailní popis

Detailní popis

The Thermo Scientific 5-Fluoroorotic Acid is a yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below). Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).

Applications

  • Detection of URA3 gene expression in yeast
  • Construction of yeast 2-hybrid libraries (see Reference 1)
  • Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements, and identification of gene mutations (see​ Reference 2)
Convertion of 5-FOA to fluorodeoxyuridine

Convertion of 5-FOA to fluorodeoxyuridine

5-Fluoroorotic Acid Chemical Structure

Note

  • Low solubility in water
  • Solubility in dimethylsulfoxide is greater than 150 mg/mL
  • Preparation of a 100 mg/mL solution in dime-thylsulfoxide is recommended
Formula C5H3FN2O4 x H2O
Hazardous No
Molecular weight 192.1
Quality Control Purity of 98%.
Storage Store at -20°C in the dark.
Storage Condition -20 C

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