Ruthenium tetroxide is very similar to Osmium tetroxide, and is used as a fixation/staining agent for electron microscopy. Ruthenium tetroxide offers excellent staining of saturated and unsaturated polymer materials as well as stabilizing thin sections under the bombardment of the electron beam.
NOTE: Penetration of ruthenium tetroxide into the tissue is poor.
- Caughey, R.C. and Miller, M.A., Proceed. of 44th Ann. Mtg. EMSA (1986), page 256
- Hayat, M.A., Basic Technique for Electron Microscopy, Academic Press, NY, 1986, p 22
- Trent, J.S., Macromolecules, 17, 2930 (1984); 16, 539 (1983)
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